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Amnis® ImageStream: Combining High Resolution Microscopy and Flow Cytometry to Advance your Discovery

Thursday October 3rd, 2019
Time: 09:00 – 17:00

Where:

Amphithéâtre Hélène Martel-Massignac

Bâtiment Biologie Du Développement – BDD

Address:

Institute Curie | Pôle de Biologie du Développement 11 

rue Pierre et Marie Curie | 75005 Paris

Planner: Institute Curie together with Luminex Corporation

 The Curie Institute Logo

The revolutionary AMNIS® ImageStream®X Mk II Imaging Flow Cytometer combines the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy. This unique combination enables a broad range of applications that would be impossible using either technique alone.



The AMNIS® ImageStreamX Mk II System is a benchtop, multispectral, imaging flow cytometer designed for the acquisition of up to 12 channels of cellular imagery. By collecting large numbers of digital images per sample and providing a numerical representation of image-based features, the AMNIS® ImageStreamX Mk II combines the per cell information content provided by standard microscopy with the statistical significance afforded by large sample sizes common to standard flow cytometry. With the AMNIS® ImageStreamX Mk II System, fluorescence intensity measurements are acquired as with a conventional flow cytometer; however, the best applications for the AMNIS® ImageStreamX Mk II take advantage of the system’s imaging abilities to locate and quantitate the distribution of signals on in or between cells.

Researchers will come and present how this technology helped them to empower their research in different areas. 

 

These products are for Research Use Only. Not for use in diagnostic procedures. Please contact Luminex at support@luminexcorp.com to obtain the appropriate product information for your country of residence.

 

09:00 Welcome Registration and Coffee
09:45

Introduction user meeting

Jan Brants, Luminex Corporation and Coralie Guérin, Institut Curie & Université de Paris

10:00

Characterization of hematopoietic cell transplant products by imaging flow cytometry

Coralie Guerin, Institut Curie & Université de Paris

10:20

Phenotypic and functional analysis of Chronic Lymphocytic Leukemia Nurse-Like Cells by Imaging Flow Cytometry 

Betty Dondi, U978, Université Paris 13

10:40

Morphological and molecular polarizations during T lymphocyte migration

Jérôme Delon, U1016, Institut Cochin, Paris

10:55

Image Flow Cytometry: From protein localization to stem cell morphology

Jéremy Cosette, Plateforme Cytométrie/Imagerie, Généthon, Paris

11:15

Kinetic of Etv3 and Etv6 translocation during human monocyte differentiation

Javiera Villar, Institut Curie

11:30

Adventures in Imaging Flow Cytometry

Ziv Porat, Weizmann Institute of Science

12:00 Lunch
13:30

Characterization of the NFAT role in RORgt gene regulation in human CD4+ T cells

Tharshana Stephen, CB UTechS, Institut Pasteur Paris

13:45

Visualisation de co-localisations intracellulaires sur des cellules épithéliales adhérentes avec l’ImageStream

Valérie Gratio, UMR 1149, Université Paris Diderot

14:00

Analyse moléculaire des marqueurs du vieillissement par Imagerie en flux ImageStreamX

Julien Cherfils, IRCAN, Nice

14:15

Amnis, pocked cells and spleen function  

Pierre Buffet, UMR_S 1134, Université Paris Diderot

14:30

Applications of imaging flow cytometry in red cell research

Sara El Hoss, UMR_S 1134, Université Paris Diderot

14:45

Single EVs and looking at EV internalization into cells   

Haley Pugsley, Amnis Luminex Corporation, Seattle

15:30

Tracking of Cardiac derived EVs by imaging cytometry in response to Myocardial Infarction       

Xavier Loyer, PARCC

15:45

Break

16:00

Caractérisation de sonde fluorescentes pour le panel design sur ImageStream

Sebastien Dussurgey, SFR Biosciences, Lyon

16:30

New software Analysis Modules - Powered by Artificial Intelligence!

Vidya Venkatachalam, Amnis Luminex Corporation, Seattle

17:30

Q&A and Closing Remarks